Microbial Consortium HJ-SH with Very High Degradation Efficiency of Phenanthrene

Phenanthrene (PHE) is one of the model compounds polycyclic aromatic hydrocarbons (PAHs). In this study, a natural PHE-degrading microbial consortium, named HJ-SH, with very high degradation efficiency was isolated from soil exposed to long-term PHE contamination. The results GC analysis showed that consortium HJ-SH degraded 98% 100 mg/L in 3 days and 93% 1000 5 days, an higher than any other consortia, even most engineered strains consortia reported so far. Seven dominating were SH-1 SH-7, which identified according morphological observation 16S rDNA sequencing as Pseudomonas sp., Stenotrophomonas Delftia Brevundimonas Curtobacterium Microbacterium respectively. Among all seven single strains, SH-4 strongest ability, had biggest contribution. However, it interesting can hold its ability only co-existence these strains. Moreover, exhibited tolerance for PHE, up 4.5 g/L, degrade some typical organic pollutants such biphenyl, anthracene, n-hexadecane ratios 93%, 92% 70%, respectively, under initial concentration days. Then, we constructed artificial HJ-7 consisting SH-7. After comparing ratios, cell growth, relative rates, concluded easier reproducibility, better application stability, larger room modification largely replace HJ-SH. conclusion, research provided novel tools new insights bioremediation using consortia.